Precise BAC targeting of genetically polymorphic mouse ES cells

The use of bacterial artificial chromosomes (BACs) provides a consistent and high targeting efficiency of homologous recombination in embryonic stem (ES) cells, facilitated by long stretches of sequence homology. Here, we introduce a BAC targeting method which employs restriction fragment length pol...

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Bibliographische Detailangaben
Hauptverfasser: Barakat, Tahsin Stefan, Rentmeester, Eveline, Sleutels, Frank, Grootegoed, J. Anton, Gribnau, Joost
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2011
Schlagworte:
Methods Online
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3185440/
https://ncbi.nlm.nih.gov/pubmed/21737430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr550
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