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Design of RNA enzymes distinguishing a single base mutation in RNA.
RNA enzymes (ribozymes) which can cleave RNA by recognizing sequences of 9-15 bases are described. Substrates must contain UX (X = U, C or A). A ribozyme consisting of two oligoribonucleotides (19 mer and 15 mer) was shown to cleave a ribo 11 mer catalytically with Km and kcat values of 0.53 microM...
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| Autores principales: | , , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
1989
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| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC318433/ https://ncbi.nlm.nih.gov/pubmed/2476725 |
| Etiquetas: |
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