A simple method for site-directed mutagenesis using the polymerase chain reaction.
We have developed a general and simple method for directing specific sequence changes in a plasmid using primed amplification by the polymerase chain reaction (PCR). The method is based on the amplification of the entire plasmid using primers that include the desired changes. The method is rapid, si...
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| Vydáno v: | Nucleic Acids Res |
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| Hlavní autoři: | , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1989
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| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318348/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2674899/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.16.6545 |
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