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Evaluation of Pulsed-FRAP and Conventional-FRAP for Determination of Protein Mobility in Prokaryotic Cells
BACKGROUND: Macromolecule mobility is often quantified with Fluorescence Recovery After Photobleaching (FRAP). Throughout literature a wide range of diffusion coefficients for GFP in the cytoplasm of Escherichia coli (3 to 14 µm(2)/s) is reported using FRAP-based approaches. In this study, we have e...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3182251/ https://ncbi.nlm.nih.gov/pubmed/21980523 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0025664 |
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