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Dual-Color Fluorescence-Burst Analysis to Probe Protein Efflux through the Mechanosensitive Channel MscL

The mechanosensitive channel protein of large conductance, MscL, from Escherichia coli has been implicated in protein efflux, but the passage of proteins through the channel has never been demonstrated. We used dual-color fluorescence-burst analysis to evaluate the efflux of fluorescent labeled comp...

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Detalhes bibliográficos
Main Authors: van den Bogaart, Geert, Krasnikov, Victor, Poolman, Bert
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1783895/
https://ncbi.nlm.nih.gov/pubmed/17142294
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.088708
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