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Dual-Color Fluorescence-Burst Analysis to Probe Protein Efflux through the Mechanosensitive Channel MscL

The mechanosensitive channel protein of large conductance, MscL, from Escherichia coli has been implicated in protein efflux, but the passage of proteins through the channel has never been demonstrated. We used dual-color fluorescence-burst analysis to evaluate the efflux of fluorescent labeled comp...

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Bibliografische gegevens
Hoofdauteurs: van den Bogaart, Geert, Krasnikov, Victor, Poolman, Bert
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Biophysical Society 2007
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1783895/
https://ncbi.nlm.nih.gov/pubmed/17142294
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.088708
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