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Chemiluminescent detection of DNA: application for DNA sequencing and hybridization.

A non-radioactive DNA detection chemistry is described and its application is shown for DNA hybridization and standard dideoxy DNA sequencing. The method employes a biotin-streptavidin system which binds an enzyme specifically to a target DNA and upon exposure to substrate, the enzyme catalyzes a ch...

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Publicat a:Nucleic Acids Res
Autors principals: Beck, S, O'Keeffe, T, Coull, J M, Köster, H
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1989
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318099/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2762122/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.13.5115
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