Cloning and characterization of the genes encoding the MspI restriction modification system.
The genes encoding the MspI restriction modification system, which recognizes the sequence 5' CCGG, have been cloned into pUC9. Selection was based on expression of the cloned methylase gene which renders plasmid DNA insensitive to MspI cleavage in vitro. Initially, an insert of 15 kb was obtained w...
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| Опубліковано в:: | Nucleic Acids Res |
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| Автори: | , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Oxford University Press
1989
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| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC317708/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2471145/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.8.3001 |
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