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A filter microplate assay for quantitative analysis of DNA-binding proteins using fluorescent DNA
We present a rapid method for quantifying the apparent DNA binding affinity and capacity of recombinant transcription factors (TFs). We capture His(6)-tagged TFs using Ni-NTA agarose and incubate the immobilized TFs with fluorescently-labeled cognate DNA probes. After washing, the strength of the fl...
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| 主要な著者: | , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2011
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3175489/ https://ncbi.nlm.nih.gov/pubmed/21447317 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.03.027 |
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