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Quantitation of secreted proteins using mCherry fusion constructs and a fluorescent microplate reader

Traditional assays for secreted proteins include methods such as Western blot or ELISA detection of the protein in the cell culture media. We describe a method for the detection of a secreted protein based on fluorescent measurement of a mCherry fusion reporter. This microplate reader-based mCherry...

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Detalhes bibliográficos
Publicado no:Anal Biochem
Main Authors: Duellman, Tyler, Burnett, John, Yang, Jay
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4500511/
https://ncbi.nlm.nih.gov/pubmed/25542417
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2014.12.010
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