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Quantitation of secreted proteins using mCherry fusion constructs and a fluorescent microplate reader
Traditional assays for secreted proteins include methods such as Western blot or ELISA detection of the protein in the cell culture media. We describe a method for the detection of a secreted protein based on fluorescent measurement of a mCherry fusion reporter. This microplate reader-based mCherry...
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| Udgivet i: | Anal Biochem |
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| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2014
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4500511/ https://ncbi.nlm.nih.gov/pubmed/25542417 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2014.12.010 |
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