Cap methyltransferase selective binding and methylation of GpppG-RNA are stimulated by importin-α

We screened a human cDNA library for proteins that bind mRNA cap methyltransferase (MT) and isolated nuclear transporter importin-α (Impα). This direct association was confirmed by glutathione S-transferase (GST) pulldown, coimmunoprecipitation, and nuclear colocalization. In gel shift assays, MT se...

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Bibliographic Details
Main Authors: Wen, Yingxia, Shatkin, Aaron J.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2000
Subjects:
Research Communication
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC317093/
https://ncbi.nlm.nih.gov/pubmed/11114884
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