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Using Superfolder Green Fluorescent Protein for Periplasmic Protein Localization Studies
Studies investigating the subcellular localization of periplasmic proteins have been hampered by problems with the export of green fluorescent protein (GFP). Here we show that a superfolding variant of GFP (sfGFP) is fluorescent following Sec-mediated transport and works best when the cotranslationa...
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| Hlavní autoři: | , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology
2011
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3165667/ https://ncbi.nlm.nih.gov/pubmed/21764912 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00315-11 |
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