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Drosophila P-element transposase is a novel site-specific endonuclease

We developed in vitro assays to study the first step of the P-element transposition reaction: donor DNA cleavage. We found that P-element transposase required both 5′ and 3′ P-element termini for efficient DNA cleavage to occur, suggesting that a synaptic complex forms prior to cleavage. Transposase...

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Detalhes bibliográficos
Main Authors: Beall, Eileen L., Rio, Donald C.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 1997
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC316450/
https://ncbi.nlm.nih.gov/pubmed/9284052
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