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Transposase makes critical contacts with, and is stimulated by, single-stranded DNA at the P element termini in vitro.
P elements transpose by a cut-and-paste mechanism. Donor DNA cleavage mediated by transposase generates 17 nucleotide (nt) 3' single-strand extensions at the P element termini which, when present on oligonucleotide substrates, stimulate both the strand-transfer and disintegration reactions in v...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1998
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1170556/ https://ncbi.nlm.nih.gov/pubmed/9524133 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/17.7.2122 |
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