ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system

We have developed an in vitro mRNA stability system using HeLa cell cytoplasmic S100 extracts and exogenous polyadenylated RNA substrates that reproduces regulated aspects of mRNA decay. The addition of cold poly(A) competitor RNA activated both a sequence-specific deadenylase activity in the extrac...

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Bibliographic Details

Published in:	Genes Dev
Main Authors:	Ford, Lance P., Watson, Janice, Keene, Jack D., Wilusz, Jeffrey
Format:	Artigo
Language:	Inglês
Published:	Cold Spring Harbor Laboratory Press 1999
Subjects:	Research Paper
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC316394/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9925643/ https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gad.13.2.188
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