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ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system
We have developed an in vitro mRNA stability system using HeLa cell cytoplasmic S100 extracts and exogenous polyadenylated RNA substrates that reproduces regulated aspects of mRNA decay. The addition of cold poly(A) competitor RNA activated both a sequence-specific deadenylase activity in the extrac...
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| Główni autorzy: | , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
Cold Spring Harbor Laboratory Press
1999
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC316394/ https://ncbi.nlm.nih.gov/pubmed/9925643 |
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