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ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system

We have developed an in vitro mRNA stability system using HeLa cell cytoplasmic S100 extracts and exogenous polyadenylated RNA substrates that reproduces regulated aspects of mRNA decay. The addition of cold poly(A) competitor RNA activated both a sequence-specific deadenylase activity in the extrac...

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書誌詳細
主要な著者: Ford, Lance P., Watson, Janice, Keene, Jack D., Wilusz, Jeffrey
フォーマット: Artigo
言語:Inglês
出版事項: Cold Spring Harbor Laboratory Press 1999
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC316394/
https://ncbi.nlm.nih.gov/pubmed/9925643
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