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Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70
BACKGROUND: Escherichia coli cell-free expression systems use bacteriophage RNA polymerases, such as T7, to synthesize large amounts of recombinant proteins. These systems are used for many applications in biotechnology, such as proteomics. Recently, informational processes have been reconstituted i...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2010
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3161345/ https://ncbi.nlm.nih.gov/pubmed/20576148 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1754-1611-4-8 |
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