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Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system

BACKGROUND: A large amount of recombinant proteins can be synthesized in a few hours with Escherichia coli cell-free expression systems based on bacteriophage transcription. These cytoplasmic extracts are used in many applications that require large-scale protein production such as proteomics and hi...

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Detalhes bibliográficos
Main Authors: Shin, Jonghyeon, Noireaux, Vincent
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2907309/
https://ncbi.nlm.nih.gov/pubmed/20594314
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1754-1611-4-9
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