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A tricistronic human adrenodoxin reductase-adrenodoxin–cytochrome P450 27A1 vector system for substrate hydroxylation in Escherichia coli

Cytochrome P450 (P450) 27A1 catalyzes 27-hydroxylation of cholesterol and 25-hydroxylation of vitamin D(3), serving as an important component for the maintenance of lipid homeostasis. In eukaryotic cells P450 27A1 is a membrane-bound protein located on the inner mitochondrial membrane and requires t...

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Détails bibliographiques
Auteurs principaux: Salamanca-Pinzón, S. Giovanna, Guengerich, F. Peter
Format: Artigo
Langue:Inglês
Publié: 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3155662/
https://ncbi.nlm.nih.gov/pubmed/21621619
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2011.05.008
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