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Biophysical Response to Pulsed Laser Microbeam-lnduced Cell Lysis and Molecular Delivery

Cell lysis and molecular delivery in confluent monolayers of PtK(2) cells are achieved by the delivery of 6 ns, λ = 532 nm laser pulses via a 40×, 0.8 NA microscope objective. With increasing distance from the point of laser focus we find regions of (a) immediate cell lysis; (b) necrotic cells that...

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Detalhes bibliográficos
Main Authors: Hellman, Amy N., Rau, Kaustubh R., Yoon, Helen H., Venugopalan, Vasan
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3155384/
https://ncbi.nlm.nih.gov/pubmed/19343632
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jbio.200710010
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