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Pulsed Laser Microbeam-Induced Cell Lysis: Time-Resolved Imaging and Analysis of Hydrodynamic Effects

Time-resolved imaging was used to examine the use of pulsed laser microbeam irradiation to produce cell lysis. Lysis was accomplished through the delivery of 6 ns, λ = 532 nm laser pulses via a 40×, 0.8 NA objective to a location 10 μm above confluent monolayers of PtK(2) cells. The process dynamics...

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Detalhes bibliográficos
Main Authors: Rau, Kaustubh R., Quinto-Su, Pedro A., Hellman, Amy N., Venugopalan, Vasan
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1479069/
https://ncbi.nlm.nih.gov/pubmed/16617076
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.079921
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