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An improved PCR strategy for fast screening of specific and random integrations in rAAV-mediated gene targeted cell clones
BACKGROUND: Gene targeting by homologous recombination using recombinant adeno-associated virus (rAAV) is becoming a useful tool for basic research and therapeutic applications due to the remarkably high targeting frequency of rAAV virus vectors. However, the screening for the pure gene-targeted and...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3154164/ https://ncbi.nlm.nih.gov/pubmed/21777457 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-4-246 |
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