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Techniques for Imaging Ca(2+) Signaling in Human Sperm
Fluorescence microscopy of cells loaded with fluorescent, Ca(2+)-sensitive dyes is used for measurement of spatial and temporal aspects of Ca(2+) signaling in live cells. Here we describe the method used in our laboratories for loading suspensions of human sperm with Ca(2+)-reporting dyes and measur...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3153901/ https://ncbi.nlm.nih.gov/pubmed/20567212 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1996 |
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