Techniques for Imaging Ca(2+) Signaling in Human Sperm

Fluorescence microscopy of cells loaded with fluorescent, Ca(2+)-sensitive dyes is used for measurement of spatial and temporal aspects of Ca(2+) signaling in live cells. Here we describe the method used in our laboratories for loading suspensions of human sperm with Ca(2+)-reporting dyes and measur...

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Bibliografiske detaljer
Main Authors: Nash, Katherine, Lefievre, Linda, Peralta-Arias, Ruben, Morris, Jennifer, Morales-Garcia, Aduen, Connolly, Tom, Costello, Sarah, Kirkman-Brown, Jackson C., Publicover, Stephen J.
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2010
Fag:
Cellular Biology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3153901/
https://ncbi.nlm.nih.gov/pubmed/20567212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1996
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