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A rapid, inexpensive yeast-based dual-fluorescence assay of programmed—1 ribosomal frameshifting for high-throughput screening

Programmed −1 ribosomal frameshifting (−1 PRF) is a mechanism that directs elongating ribosomes to shift-reading frame by 1 base in the 5′ direction that is utilized by many RNA viruses. Importantly, rates of −1 PRF are fine-tuned by viruses, including Retroviruses, Coronaviruses, Flavivriuses and i...

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Detalhes bibliográficos
Main Authors: Rakauskaitė, Rasa, Liao, Pei-Yu, Rhodin, Michael H. J., Lee, Kelvin, Dinman, Jonathan D.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2011
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3152369/
https://ncbi.nlm.nih.gov/pubmed/21602263
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr382
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