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Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

In this protocol we describe the use of Fluorescent Speckle Microscopy (FSM) to capture high-resolution images of actin dynamics in PtK1 cells. A unique advantage of FSM is its ability to capture the movement and turnover kinetics (assembly/disassembly) of the F-actin network within living cells. Th...

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Sparad:
Bibliografiska uppgifter
Huvudupphovsmän: Lim, James, Danuser, Gaudenz
Materialtyp: Artigo
Språk:Inglês
Publicerad: MyJove Corporation 2009
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3149907/
https://ncbi.nlm.nih.gov/pubmed/19684563
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1325
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