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Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
In this protocol we describe the use of Fluorescent Speckle Microscopy (FSM) to capture high-resolution images of actin dynamics in PtK1 cells. A unique advantage of FSM is its ability to capture the movement and turnover kinetics (assembly/disassembly) of the F-actin network within living cells. Th...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3149907/ https://ncbi.nlm.nih.gov/pubmed/19684563 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1325 |
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