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Simultaneous mapping of filamentous actin flow and turnover in migrating cells by quantitative fluorescent speckle microscopy

We report advances in quantitative fluorescent speckle microscopy to generate simultaneous maps of cytoskeleton flow and rates of net assembly and disassembly in living cells. We apply this tool to analyze the filamentous actin (F-actin) dynamics at the front of migrating cells. F-actin turnover and...

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Autors principals: Vallotton, Pascal, Gupton, Stephanie L., Waterman-Storer, Clare M., Danuser, Gaudenz
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC470731/
https://ncbi.nlm.nih.gov/pubmed/15210979
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0300552101
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