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Efficient Direct Extended-Spectrum β-Lactamase Detection by Multiplex Real-Time PCR: Accurate Assignment of Phenotype by Use of a Limited Set of Genetic Markers

The number and diversity of genes potentially complicate genetic approaches to the rapid detection of transmissible extended-spectrum β-lactamase genes. We developed a robust multiplexed real-time PCR assay based on targets identified in a prior survey and used this to detect relevant genes in 617 c...

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Detalhes bibliográficos
Main Authors: Ellem, Justin, Partridge, Sally R., Iredell, Jonathan R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3147779/
https://ncbi.nlm.nih.gov/pubmed/21613435
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02647-10
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