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A large-scale method to measure absolute protein phosphorylation stoichiometries

The underlying functional role of protein phosphorylation is impacted by its fractional stoichiometry. Thus, a comprehensive strategy to study phosphorylation dynamics should include an assessment of site stoichiometry. Here, we developed an integrated method that relies on phosphatase treatment and...

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Bibliographic Details
Main Authors: Wu, Ronghu, Haas, Wilhelm, Dephoure, Noah, Huttlin, Edward L., Zhai, Bo, Sowa, Mathew E., Gygi, Steven P.
Format: Artigo
Language:Inglês
Published: 2011
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3146562/
https://ncbi.nlm.nih.gov/pubmed/21725298
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1636
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