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A large-scale method to measure absolute protein phosphorylation stoichiometries
The underlying functional role of protein phosphorylation is impacted by its fractional stoichiometry. Thus, a comprehensive strategy to study phosphorylation dynamics should include an assessment of site stoichiometry. Here, we developed an integrated method that relies on phosphatase treatment and...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3146562/ https://ncbi.nlm.nih.gov/pubmed/21725298 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1636 |
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