Highly Efficient Production of Soluble Proteins from Insoluble Inclusion Bodies by a Two-Step-Denaturing and Refolding Method

The production of recombinant proteins in a large scale is important for protein functional and structural studies, particularly by using Escherichia coli over-expression systems; however, approximate 70% of recombinant proteins are over-expressed as insoluble inclusion bodies. Here we presented an...

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Main Authors: Yang, Zhong, Zhang, Linlin, Zhang, Yan, Zhang, Ting, Feng, Yanye, Lu, Xiuxiu, Lan, Wenxian, Wang, Jufang, Wu, Houming, Cao, Chunyang, Wang, Xiaoning
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3146519/
https://ncbi.nlm.nih.gov/pubmed/21829569
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0022981
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