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Cloning and Expression of Randomly Mutated Bacillus subtilis α-Amylase Genes in HB101
The aim of this study was to isolate and express the randomly mutated α-amylase gene from B. subtilis strain 168. BS168F: 5′-gtgtcaagaatgtttgc-3′ and BS168R: 3′-gttttgttaaaagatga-5′ primers were used to amplify the amylase gene using the following cycle in error-prone PCR method: 94°C for 30 s, 40°C...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
SAGE-Hindawi Access to Research
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3145346/ https://ncbi.nlm.nih.gov/pubmed/21808739 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4061/2011/305956 |
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