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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

A detailed protocol for crystallizing membrane proteins by using lipidic mesophases is described. This method has variously been referred to as the lipidic cubic phase or in meso method. The method has been shown to be quite versatile in that it has been used to solve X-ray crystallographic structur...

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Detalhes bibliográficos
Main Authors: Caffrey, Martin, Porter, Christopher
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3144658/
https://ncbi.nlm.nih.gov/pubmed/21113125
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1712
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