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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

A detailed protocol for crystallizing membrane proteins by using lipidic mesophases is described. This method has variously been referred to as the lipidic cubic phase or in meso method. The method has been shown to be quite versatile in that it has been used to solve X-ray crystallographic structur...

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Bibliographic Details
Main Authors: Caffrey, Martin, Porter, Christopher
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2010
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3144658/
https://ncbi.nlm.nih.gov/pubmed/21113125
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1712
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