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Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

An important route to understanding how proteins function at a mechanistic level is to have the structure of the target protein available, ideally at atomic resolution. Presently, there is only one way to capture such information as applied to integral membrane proteins (Figure 1), and the complexes...

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Detalhes bibliográficos
Main Authors: Li, Dianfan, Boland, Coilín, Aragao, David, Walsh, Kilian, Caffrey, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3485064/
https://ncbi.nlm.nih.gov/pubmed/22971942
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/4001
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