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Fluorescence based evaluation of shRNA efficacy

RNA interference is a cellular mechanism regulating levels of mRNAs. It has been widely exploited to knockdown specific protein targets. The selected interfering RNA sequence greatly influences its ability to knockdown the target. Here we present a method for constructing multiple testing plasmids w...

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Bibliografiske detaljer
Main Authors: Naughton, B.J., Han, Dawn D., Gu, Howard H.
Format: Artigo
Sprog:Inglês
Udgivet: 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3143307/
https://ncbi.nlm.nih.gov/pubmed/21726522
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.06.008
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