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A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells

Although there are several inducible expression systems for mammalian cells, the most reliable one is the tetracycline-regulated expression system. This system is well-established and widely used by many researchers. Although Clontech provides several types of cells that stably express reverse tetra...

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Autors principals: Iwaki, Takayuki, Umemura, Kazuo
Format: Artigo
Idioma:Inglês
Publicat: Springer Netherlands 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3140840/
https://ncbi.nlm.nih.gov/pubmed/21553115
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-011-9357-6
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