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A ring-opening mechanism for DNA binding in the central channel of the T7 helicase–primase protein
We have investigated the mechanism of binding single-stranded DNA (ssDNA) into the central channel of the ring-shaped T7 gp4A′ helicase–primase hexamer. Presteady-state kinetic studies show a facilitated five-step mechanism and provide understanding of how a ring-shaped helicase can be loaded on the...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC313936/ https://ncbi.nlm.nih.gov/pubmed/10880454 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/19.13.3418 |
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