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Glucan-binding domain of a glucosyltransferase from Streptococcus sobrinus: isolation of a 55-kilodalton peptide from a trypsin digest of glucosyltransferase prebound to insoluble glucan.

We isolated a glucan-binding domain of water-insoluble glucan synthase (GTF-I) of Streptococcus sobrinus B13. Mild trypsin digestion of GTF-I bound to a water-insoluble glucan (IG) produced one predominant large fragment (55 kilodaltons). The fragment was easily recovered in IG precipitate. The isol...

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Autori principali: Kobayashi, S, Koga, K, Hayashida, O, Nakano, Y, Hasegawa, Y
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1989
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC313862/
https://ncbi.nlm.nih.gov/pubmed/2525114
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