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Glucan-binding domain of a glucosyltransferase from Streptococcus sobrinus: isolation of a 55-kilodalton peptide from a trypsin digest of glucosyltransferase prebound to insoluble glucan.

We isolated a glucan-binding domain of water-insoluble glucan synthase (GTF-I) of Streptococcus sobrinus B13. Mild trypsin digestion of GTF-I bound to a water-insoluble glucan (IG) produced one predominant large fragment (55 kilodaltons). The fragment was easily recovered in IG precipitate. The isol...

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Detalhes bibliográficos
Publicado no:Infect Immun
Principais autores: Kobayashi, S, Koga, K, Hayashida, O, Nakano, Y, Hasegawa, Y
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC313862/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2525114/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.57.7.2210-2213.1989
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