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Isolation of Listeria monocytogenes small-plaque mutants defective for intracellular growth and cell-to-cell spread.

To dissect the regulatory and structural requirements for Listeria monocytogenes intracellular growth and cell-to-cell spread, we designed a protocol based on transposon mutagenesis and the isolation of mutants which form small plaques in monolayers of mouse L2 cell fibroblasts. Two different transp...

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Autors principals: Sun, A N, Camilli, A, Portnoy, D A
Format: Artigo
Idioma:Inglês
Publicat: 1990
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC313727/
https://ncbi.nlm.nih.gov/pubmed/2172168
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