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Isolation of Listeria monocytogenes small-plaque mutants defective for intracellular growth and cell-to-cell spread.

To dissect the regulatory and structural requirements for Listeria monocytogenes intracellular growth and cell-to-cell spread, we designed a protocol based on transposon mutagenesis and the isolation of mutants which form small plaques in monolayers of mouse L2 cell fibroblasts. Two different transp...

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Detalhes bibliográficos
Publicado no:Infect Immun
Principais autores: Sun, A N, Camilli, A, Portnoy, D A
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1990
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC313727/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2172168/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.58.11.3770-3778.1990
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