Abundant Non-canonical dUTP Found in Primary Human Macrophages Drives Its Frequent Incorporation by HIV-1 Reverse Transcriptase

Terminally differentiated/non-dividing macrophages contain extremely low cellular dNTP concentrations (20–40 nm), compared with activated CD4(+) T cells (2–5 μm). However, our LC-MS/MS study revealed that the non-canonical dUTP concentration (2.9 μm) is ∼60 times higher than TTP in macrophages, wher...

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Bibliographic Details
Main Authors: Kennedy, Edward M., Daddacha, Waaqo, Slater, Rebecca, Gavegnano, Christina, Fromentin, Emilie, Schinazi, Raymond F., Kim, Baek
Format: Artigo
Language:Inglês
Published: American Society for Biochemistry and Molecular Biology 2011
Subjects:
DNA and Chromosomes
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3137078/
https://ncbi.nlm.nih.gov/pubmed/21454906
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.234047
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