Clinical Microfluidics for Neutrophil Genomics and Proteomics

Neutrophils play critical roles in modulating the immune response. We present a robust methodology for rapidly isolating neutrophils directly from whole blood and develop ‘on-chip’ processing for mRNA and protein isolation for genomics and proteomics. We validate this device with an ex vivo stimulat...

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Hauptverfasser: Kotz, Kenneth T., Xiao, Wenzong, Miller-Graziano, Carol, Qian, Wei-Jun, Russom, Aman, Warner, Elizabeth A., Moldawer, Lyle L., De, Asit, Bankey, Paul E., Petritis, Brianne O., Camp, David G., Rosenbach, Alan E., Goverman, Jeremy, Fagan, Shawn P., Brownstein, Bernard H., Irimia, Daniel, Xu, Weihong, Wilhelmy, Julie, Mindrinos, Michael N., Smith, Richard D., Davis, Ronald W., Tompkins, Ronald G., Toner, Mehmet
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3136804/
https://ncbi.nlm.nih.gov/pubmed/20802500
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nm.2205
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