Clinical Microfluidics for Neutrophil Genomics and Proteomics

Neutrophils play critical roles in modulating the immune response. We present a robust methodology for rapidly isolating neutrophils directly from whole blood and develop ‘on-chip’ processing for mRNA and protein isolation for genomics and proteomics. We validate this device with an ex vivo stimulat...

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Detalhes bibliográficos
Main Authors: Kotz, Kenneth T., Xiao, Wenzong, Miller-Graziano, Carol, Qian, Wei-Jun, Russom, Aman, Warner, Elizabeth A., Moldawer, Lyle L., De, Asit, Bankey, Paul E., Petritis, Brianne O., Camp, David G., Rosenbach, Alan E., Goverman, Jeremy, Fagan, Shawn P., Brownstein, Bernard H., Irimia, Daniel, Xu, Weihong, Wilhelmy, Julie, Mindrinos, Michael N., Smith, Richard D., Davis, Ronald W., Tompkins, Ronald G., Toner, Mehmet
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3136804/
https://ncbi.nlm.nih.gov/pubmed/20802500
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nm.2205
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