Protein Stability and Folding Kinetics in the Nucleus and Endoplasmic Reticulum of Eucaryotic Cells

We measure the stability and folding relaxation rate of phosphoglycerate kinase (PGK) Förster resonance energy transfer (FRET) constructs localized in the nucleus or in the endoplasmic reticulum (ER) of eukaryotic cells. PGK has a more compact native state in the cellular compartments than in aqueou...

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Detalhes bibliográficos
Main Authors: Dhar, A., Girdhar, K., Singh, D., Gelman, H., Ebbinghaus, S., Gruebele, M.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2011
Assuntos:
Protein
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3136782/
https://ncbi.nlm.nih.gov/pubmed/21767495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.05.071
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