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GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules

Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic DNA parts in a standardized way. Here we present GoldenBraid (GB), a standardized assembly system based on type IIS restriction enzymes that allows the indefi...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Sarrion-Perdigones, Alejandro, Falconi, Erica Elvira, Zandalinas, Sara I., Juárez, Paloma, Fernández-del-Carmen, Asun, Granell, Antonio, Orzaez, Diego
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3131274/
https://ncbi.nlm.nih.gov/pubmed/21750718
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0021622
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