A modular toolbox for gRNA–Cas9 genome engineering in plants based on the GoldenBraid standard

BACKGROUND: The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the construction of transcriptional gene circuits, many of which depend on the technical abili...

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Vydáno v:Plant Methods
Hlavní autoři: Vazquez-Vilar, Marta, Bernabé-Orts, Joan Miquel, Fernandez-del-Carmen, Asun, Ziarsolo, Pello, Blanca, Jose, Granell, Antonio, Orzaez, Diego
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2016
Témata:
Methodology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4736081/
https://ncbi.nlm.nih.gov/pubmed/26839579
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-016-0101-2
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