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Manufacturing DNA microarrays of high spot homogeneity and reduced background signal

Analyses on DNA microarrays depend considerably on spot quality and a low background signal of the glass support. By using betaine as an additive to a spotting solution made of saline sodium citrate, both the binding efficiency of spotted PCR products and the homogeneity of the DNA spots is improved...

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Détails bibliographiques
Auteurs principaux: Diehl, Frank, Grahlmann, Susanne, Beier, Markus, Hoheisel, Jörg D.
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2001
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC31299/
https://ncbi.nlm.nih.gov/pubmed/11266573
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