Strategy for Robust Detection of Insertions, Deletions, and Point Mutations in CEBPA, a GC-Rich Content Gene, Using 454 Next-Generation Deep-Sequencing Technology

CEBPA mutations are of prognostic relevance in acute myeloid leukemia (AML) and are currently detected using a combination of denaturing high-performance liquid chromatography (DHPLC), gene scan/fragment length analysis, and direct Sanger sequencing. Next-generation deep pyrosequencing, principally,...

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Detalhes bibliográficos
Main Authors: Grossmann, Vera, Schnittger, Susanne, Schindela, Sonja, Klein, Hans-Ulrich, Eder, Christiane, Dugas, Martin, Kern, Wolfgang, Haferlach, Torsten, Haferlach, Claudia, Kohlmann, Alexander
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Investigative Pathology 2011
Assuntos:
Regular Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3128616/
https://ncbi.nlm.nih.gov/pubmed/21354046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmoldx.2010.09.001
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