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Engineering Escherichia coli BL21(DE3) Derivative Strains To Minimize E. coli Protein Contamination after Purification by Immobilized Metal Affinity Chromatography

Recombinant His-tagged proteins expressed in Escherichia coli and purified by immobilized metal affinity chromatography (IMAC) are commonly coeluted with native E. coli proteins, especially if the recombinant protein is expressed at a low level. The E. coli contaminants display high affinity to diva...

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Detalhes bibliográficos
Main Authors: Robichon, Carine, Luo, Jianying, Causey, Thomas B., Benner, Jack S., Samuelson, James C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3127686/
https://ncbi.nlm.nih.gov/pubmed/21602383
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00119-11
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