Validation of Internal Controls for Extraction and Amplification of Nucleic Acids from Enteric Viruses in Water Samples

Inhibitors that reduce viral nucleic acid extraction efficiency and interfere with cDNA synthesis and/or polymerase activity affect the molecular detection of viruses in aquatic environments. To overcome these significant problems, we developed a methodology for assessing nucleic acid yields and DNA...

Full description

Saved in:
Bibliographic Details
Main Authors: Hata, Akihiko, Katayama, Hiroyuki, Kitajima, Masaaki, Visvanathan, Chettiyappan, Nol, Chea, Furumai, Hiroaki
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2011
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3127680/
https://ncbi.nlm.nih.gov/pubmed/21602369
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00077-11
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items