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Validation of Internal Controls for Extraction and Amplification of Nucleic Acids from Enteric Viruses in Water Samples

Inhibitors that reduce viral nucleic acid extraction efficiency and interfere with cDNA synthesis and/or polymerase activity affect the molecular detection of viruses in aquatic environments. To overcome these significant problems, we developed a methodology for assessing nucleic acid yields and DNA...

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Detalhes bibliográficos
Main Authors: Hata, Akihiko, Katayama, Hiroyuki, Kitajima, Masaaki, Visvanathan, Chettiyappan, Nol, Chea, Furumai, Hiroaki
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3127680/
https://ncbi.nlm.nih.gov/pubmed/21602369
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00077-11
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