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A Ribosomal Misincorporation of Lys for Arg in Human Triosephosphate Isomerase Expressed in Escherichia coli Gives Rise to Two Protein Populations

We previously observed that human homodimeric triosephosphate isomerase (HsTIM) expressed in Escherichia coli and purified to apparent homogeneity exhibits two significantly different thermal transitions. A detailed exploration of the phenomenon showed that the preparations contain two proteins; one...

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Detalhes bibliográficos
Main Authors: Aguirre, Beatriz, Costas, Miguel, Cabrera, Nallely, Mendoza-Hernández, Guillermo, Helseth, Donald L., Fernández, Paulette, Tuena de Gómez-Puyou, Marietta, Pérez-Montfort, Ruy, Torres-Larios, Alfredo, Gómez Puyou, Armando
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3125179/
https://ncbi.nlm.nih.gov/pubmed/21738601
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0021035
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